Your load factors seem to be awfully low ...
- how many frequencies per week you operate on this route? make it 7x week MINIMUM
- are the flights flown during daytime?
if both are fine, then:
- prices are probably way too high.
Actually.. Please look at the passenger demand. You are offering 10x more seats what the suspected demand for that sector is! So better switch to another route ...
Also to answer the question. The actual fuel used on each individual sector varies a bit as there may be different flight or taxi routings or different runways in use, and so on.